Nippon Genetics FastGene HIFI 2x HS Master Mix - 100 Reactions

The high fidelity of the FastGene® HiFi HS (Hot Start) Polymerase is based on the improved 3′-5′ exonuclease activity, which significantly reduces the error rate of the enzyme and makes it around 100 times more accurate than a Taq DNA polymerase. This is ideal for applications where high fidelity is essential, such as sequencing, cloning and site-directed mutagenesis.

The FastGene® HiFi HS Polymerase uses a special technology with an aptamer-like molecule that gives the polymerase hot start activity that is different from the antibody-mediated hot start normally used. The aptamer-like molecule reversibly binds and inhibits the 3′-5′ exonuclease and 5′-3′ polymerase activities of the enzyme at temperatures below 40°C. This sophisticated hot start molecule prevents primer dimer formation and non-specific amplification. It therefore maximises the sensitivity and specificity of your PCR and allows easy experimental set-up at room temperature. The FastGene® HiFi Polymerase is provided in a 2x Master Mix, which significantly speeds up the preparation of a PCR. It contains an advanced buffering system with dNTPs, Mg²⁺, reaction enhancers and the polymerase enzyme.

• Reliable high fidelity polymerase – 100x higher fidelity than Taq DNA polymerase
• Special hot start technology for maximum sensitivity and specificity
• Superior performance under difficult conditions, e.g., AT or GC-rich sequences and long templates (up to 17.5kb)
• Perfect for multiplex-PCRs and applications where high accuracy is required (e.g., cloning, site-directed mutagenesis, sequencing)
• Ready-to-use cocktail with all components necessary for a wide range of targets and fragment sizes
• 3′-5′ exonuclease and proofreading activity
• Easy experimental set-up at room temperature
• Stability of the reaction mixture for up to 24 hours before and after the PCR run
• Engineered in a way that it can amplify particularly long templates (up to 17.5kb) with a high sequence accuracy, furthermore, it shows a significant improvement in extension times (10 to 30 sec per kb), while generating high yields, even with difficult templates